pe conjugated ter119 Search Results


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R&D Systems pe conjugated ter119
(A) H&E staining of liver biopsies at the time of diagnosis of biliary atresia shows variable population of extramedullary cells (absent, scarce, or extensive cell clusters). Scale bar: 20 μM. (B) Immunofluorescence staining of surface markers, CD235a and CD71, identifies erythroblasts among hematopoietic cells. Scale bar: 20 μM. (C) Immunohistochemical staining using anti-CD71 antibody shows similar features in livers of a human neonate and newborn mice. The human liver tissue was obtained at the time of autopsy of a neonate without liver disease, and the mouse livers were obtained from saline- or RRV-injected neonatal mice at 1 day old. Scale bar: 20 μM. (D and E) Quantification of hepatic erythroblasts in response to RRV infection by using flow cytometry after incubation with anti-CD71 and <t>anti-TER119</t> antibodies at days 1, 3, and 7 after injection of RRV or saline. *P < 0.05, ***P < 0.001. Differences in mean values were statistically analyzed by 2-tailed Student’s t test. n = 3–4/group.
Pe Conjugated Ter119, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pe+conjugated+ter119/Mouse+TER-119+PE-conjugated+Antibody/pmc07308060-238-33-36
Average 90 stars, based on 1 article reviews
pe conjugated ter119 - by Bioz Stars, 2026-09
90/100 stars
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The Mouse TER-119 PE-conjugated Antibody from R&D Systems is a TER-119 antibody to TER-119. This antibody reacts with Mouse. The TER-119 antibody has been validated for the following applications: Flow Cytometry.
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Image Search Results


(A) H&E staining of liver biopsies at the time of diagnosis of biliary atresia shows variable population of extramedullary cells (absent, scarce, or extensive cell clusters). Scale bar: 20 μM. (B) Immunofluorescence staining of surface markers, CD235a and CD71, identifies erythroblasts among hematopoietic cells. Scale bar: 20 μM. (C) Immunohistochemical staining using anti-CD71 antibody shows similar features in livers of a human neonate and newborn mice. The human liver tissue was obtained at the time of autopsy of a neonate without liver disease, and the mouse livers were obtained from saline- or RRV-injected neonatal mice at 1 day old. Scale bar: 20 μM. (D and E) Quantification of hepatic erythroblasts in response to RRV infection by using flow cytometry after incubation with anti-CD71 and anti-TER119 antibodies at days 1, 3, and 7 after injection of RRV or saline. *P < 0.05, ***P < 0.001. Differences in mean values were statistically analyzed by 2-tailed Student’s t test. n = 3–4/group.

Journal: JCI Insight

Article Title: Regulation of bile duct epithelial injury by hepatic CD71 + erythroid cells

doi: 10.1172/jci.insight.135751

Figure Lengend Snippet: (A) H&E staining of liver biopsies at the time of diagnosis of biliary atresia shows variable population of extramedullary cells (absent, scarce, or extensive cell clusters). Scale bar: 20 μM. (B) Immunofluorescence staining of surface markers, CD235a and CD71, identifies erythroblasts among hematopoietic cells. Scale bar: 20 μM. (C) Immunohistochemical staining using anti-CD71 antibody shows similar features in livers of a human neonate and newborn mice. The human liver tissue was obtained at the time of autopsy of a neonate without liver disease, and the mouse livers were obtained from saline- or RRV-injected neonatal mice at 1 day old. Scale bar: 20 μM. (D and E) Quantification of hepatic erythroblasts in response to RRV infection by using flow cytometry after incubation with anti-CD71 and anti-TER119 antibodies at days 1, 3, and 7 after injection of RRV or saline. *P < 0.05, ***P < 0.001. Differences in mean values were statistically analyzed by 2-tailed Student’s t test. n = 3–4/group.

Article Snippet: Fluorochrome-conjugated mouse antibodies were as follows: FITC-conjugated CD71 from eBioscience; APC-conjugated Cd8a and Pan-NK (CD49b) from Miltenyi Biotec; PerCP-conjugated CD3, CD4, CD49b, and anti-NKp46 antibody and BV605-conjugated CD3 and CD45 from BioLegend; and PE-conjugated TER119 from R&D Systems. (catalog FAB1125P).

Techniques: Staining, Biomarker Discovery, Immunofluorescence, Immunohistochemical staining, Saline, Injection, Infection, Flow Cytometry, Incubation

(A and B) Flow cytometry plots and graphical quantification of CD71+Ter119+ erythroblasts after anti-CD71 antibody or rat IgG injection within 12 hours of birth. Livers or extrahepatic bile ducts (EHBD) were harvested at day 1 and day 3 after injection. **P < 0.01, ***P < 0.001, ****P < 0.0001, n = 4 for each group. (C) Weight, jaundice, and survival percentage after the administration of anti-CD71 antibody or isotype (rat IgG) into newborn mice, followed by the i.p. administration of RRV 24 hours later. Two-tailed Student’s t test was used for weight comparison, and χ2 test was used for jaundice percentage. *P < 0.05, ***P < 0.001, ****P < 0.0001; Kaplan-Meier survival curve was analyzed by the log-rank (Mantel-Cox) test; n = 16 for Rat IgG group and n = 21 for anti-CD71 antibody group. (D) H&E staining of liver and EHBD at days 7 and 11 after RRV infection. n = 5 for rat IgG–treated group and n = 14 for anti-CD71 antibody–treated group. PV, portal vein. Scale bar: 20 μM.

Journal: JCI Insight

Article Title: Regulation of bile duct epithelial injury by hepatic CD71 + erythroid cells

doi: 10.1172/jci.insight.135751

Figure Lengend Snippet: (A and B) Flow cytometry plots and graphical quantification of CD71+Ter119+ erythroblasts after anti-CD71 antibody or rat IgG injection within 12 hours of birth. Livers or extrahepatic bile ducts (EHBD) were harvested at day 1 and day 3 after injection. **P < 0.01, ***P < 0.001, ****P < 0.0001, n = 4 for each group. (C) Weight, jaundice, and survival percentage after the administration of anti-CD71 antibody or isotype (rat IgG) into newborn mice, followed by the i.p. administration of RRV 24 hours later. Two-tailed Student’s t test was used for weight comparison, and χ2 test was used for jaundice percentage. *P < 0.05, ***P < 0.001, ****P < 0.0001; Kaplan-Meier survival curve was analyzed by the log-rank (Mantel-Cox) test; n = 16 for Rat IgG group and n = 21 for anti-CD71 antibody group. (D) H&E staining of liver and EHBD at days 7 and 11 after RRV infection. n = 5 for rat IgG–treated group and n = 14 for anti-CD71 antibody–treated group. PV, portal vein. Scale bar: 20 μM.

Article Snippet: Fluorochrome-conjugated mouse antibodies were as follows: FITC-conjugated CD71 from eBioscience; APC-conjugated Cd8a and Pan-NK (CD49b) from Miltenyi Biotec; PerCP-conjugated CD3, CD4, CD49b, and anti-NKp46 antibody and BV605-conjugated CD3 and CD45 from BioLegend; and PE-conjugated TER119 from R&D Systems. (catalog FAB1125P).

Techniques: Flow Cytometry, Injection, Two Tailed Test, Comparison, Staining, Infection

(A–C) Representative plots and quantification of CD71+Ter119+ cells (A), CD4+ T cells and CD8+ T cells (B), and activation marker NKp46 in CD3–CD49b+ cells and total NK cell counts (C) in livers of RRV-infected mice pretreated with either rat IgG or anti-CD71 antibody. Differences in mean values analyzed by 2-tailed Student’s t test; **P < 0.01; ****P < 0.0001; n = 4/group.

Journal: JCI Insight

Article Title: Regulation of bile duct epithelial injury by hepatic CD71 + erythroid cells

doi: 10.1172/jci.insight.135751

Figure Lengend Snippet: (A–C) Representative plots and quantification of CD71+Ter119+ cells (A), CD4+ T cells and CD8+ T cells (B), and activation marker NKp46 in CD3–CD49b+ cells and total NK cell counts (C) in livers of RRV-infected mice pretreated with either rat IgG or anti-CD71 antibody. Differences in mean values analyzed by 2-tailed Student’s t test; **P < 0.01; ****P < 0.0001; n = 4/group.

Article Snippet: Fluorochrome-conjugated mouse antibodies were as follows: FITC-conjugated CD71 from eBioscience; APC-conjugated Cd8a and Pan-NK (CD49b) from Miltenyi Biotec; PerCP-conjugated CD3, CD4, CD49b, and anti-NKp46 antibody and BV605-conjugated CD3 and CD45 from BioLegend; and PE-conjugated TER119 from R&D Systems. (catalog FAB1125P).

Techniques: Activation Assay, Marker, Infection

(A) Schematic diagram of the injection timeline for anti-CD71 antibodies or rat IgG isotypes into newborn mice. (B) The quantification of hepatic CD71+Ter119+ cells by flow cytometry shows the prevention of the 3-day surge after RRV infection. Data were analyzed by 1-way ANOVA; ***P < 0.001; ****P < 0.0001; n = 4/group. (C) Jaundice and survival differences in 3 groups. Kaplan-Meier survival curve were analyzed by log-rank (Mantel-Cox) test; *P < 0.05; **P < 0.01; n = 8-16 in each group. (D) CD4+ T cells, CD8+ T cells, and NK cells were quantified by flow cytometry. Data were analyzed by 1-way ANOVA; *P < 0.05; **P < 0.01; ***P < 0.001; n = 3–4/group.

Journal: JCI Insight

Article Title: Regulation of bile duct epithelial injury by hepatic CD71 + erythroid cells

doi: 10.1172/jci.insight.135751

Figure Lengend Snippet: (A) Schematic diagram of the injection timeline for anti-CD71 antibodies or rat IgG isotypes into newborn mice. (B) The quantification of hepatic CD71+Ter119+ cells by flow cytometry shows the prevention of the 3-day surge after RRV infection. Data were analyzed by 1-way ANOVA; ***P < 0.001; ****P < 0.0001; n = 4/group. (C) Jaundice and survival differences in 3 groups. Kaplan-Meier survival curve were analyzed by log-rank (Mantel-Cox) test; *P < 0.05; **P < 0.01; n = 8-16 in each group. (D) CD4+ T cells, CD8+ T cells, and NK cells were quantified by flow cytometry. Data were analyzed by 1-way ANOVA; *P < 0.05; **P < 0.01; ***P < 0.001; n = 3–4/group.

Article Snippet: Fluorochrome-conjugated mouse antibodies were as follows: FITC-conjugated CD71 from eBioscience; APC-conjugated Cd8a and Pan-NK (CD49b) from Miltenyi Biotec; PerCP-conjugated CD3, CD4, CD49b, and anti-NKp46 antibody and BV605-conjugated CD3 and CD45 from BioLegend; and PE-conjugated TER119 from R&D Systems. (catalog FAB1125P).

Techniques: Injection, Flow Cytometry, Infection